6.2: Análisis estructural de moléculas de ADN etiquetadas por pulsos
- Page ID
- 58509
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Cuadro 6.1. Aparición de radiomarca en fragmentos de restricción de moléculas de ADN de SV40 completadas. La cantidad relativa de marca de pulso de cada fragmento de restricción se da a continuación (la cantidad relativa de marca de pulso es la relación 3H/32P de cada fragmento, corregida para el contenido de timidina y normalizada a 1 para el fragmento A).
| Cantidad relativa de etiqueta de pulso | |||||
|---|---|---|---|---|---|
| Fragmento | 5 min | 10 min | 15 min | ||
| A | 1.0 | 1.0 | 1.0 | ||
| B | 3.9 | 3.0 | 2.3 | ||
| C | 0 | 0.75 | 0.75 | ||
| D | 0.92 | 0.86 | 1.1 | ||
| E | 1.8 | 2.0 | 1.7 | ||
| F | 4.0 | 3.1 | 2.4 | ||
| G | 5.4 | 4.2 | 2.6 | ||
| H | 1.7 | 2.5 | 2.0 | ||
| I | 2.7 | 3.0 | 2.2 | ||
| J | 4.9 | 3.7 | 2.6 | ||
| K | 2.4 | 2.9 | 1.9 | ||
Pregunta 6.2: ¿Cuál sería el patrón para la replicación unidireccional?
Pregunta 6.3: ¿Cuál sería el patrón si hubiera dos orígenes, digamos en los fragmentos E y H, con replicación bidireccional de cada uno?