6.3: Geles bidimensionales para analizar el número y posición de los orígenes de replicación
- Page ID
- 58541
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Pregunta 6.4. Se muestra un mapa de restricción para una porción de un cromosoma a continuación, junto con los patrones en geles bidimensionales para los intermedios de replicación formados por cada fragmento. ¿Dónde están los orígenes y los termini? ¿Se puede deducir la dirección del movimiento de la horquilla de replicación?
Pregunta 6.5. ¿Cómo se puede calcular la posición de un origen dentro de un fragmento de ADN a partir de un patrón asimétrico de horquilla/burbujas en un gel 2-D de moléculas de ADN replicantes?