3.1.6: Suministros para Carga/Funcionamiento de Gel

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Page ID: 57433

Nathan Reyna, Ruth Plymale, and Kristen Johnson
Ouachita Babtist University and University of New Hampshire

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Carga y Funcionamiento del Gel:

Materiales	Monto Necesario (Por reacción)
Agarosa	Varía dependiendo del porcentaje de gel
1x TBE	Aproximadamente 165 mL (para hacer gel y para plataforma de gel)
SybrGreen, SybrSafe o GelRed (Cat#: S33102)	2-3 uL
ADN digerido	5 uL
Escalera de ADN (Escalera de Carga Rapida Cat#: N0550S)	5 uL
Tinte de Carga de ADN 6x (Cat #: B7024S)	1 uL
Microondas	1 (usado por clase)
Frasco	1 (al menos 50 mL de tamaño)
Micropipeta y Puntas	1-10 uL
Plataforma de gel (MiniOne; Cat #s: M1000, M1002, M1010, M1012)	1 (con todas las piezas necesarias incluidas con la plataforma)
Bandeja de Pesaje	1
Escala	1 (usado por clase)

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