Note that this is just one of the many ways that ATP is used as a energy storage unit: in order to make a high energy acyl phosphate molecule from a low energy carboxylate, the cell must ‘spend’ the e...Note that this is just one of the many ways that ATP is used as a energy storage unit: in order to make a high energy acyl phosphate molecule from a low energy carboxylate, the cell must ‘spend’ the energy of one ATP molecule. The hydrolysis reaction is happening in the same enzyme active site – as the NH 3 is expelled in the hydrolysis of glutamine, it immediately turns around and acts as the nucleophile in the conversion of aspartyl-AMP to asparagine: