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    • https://espanol.libretexts.org/Quimica/Libro%3A_Qu%C3%ADmica_Org%C3%A1nica_con_%C3%A9nfasis_Biol%C3%B3gico_(Soderberg)/12%3A_Reacciones_de_sustitucion_de_acilo/12.3%3A_Fosfatos_de_acilo_como_%C3%A1cidos_carbox%C3%ADlicos_activados
      Note that this is just one of the many ways that ATP is used as a energy storage unit: in order to make a high energy acyl phosphate molecule from a low energy carboxylate, the cell must ‘spend’ the e...Note that this is just one of the many ways that ATP is used as a energy storage unit: in order to make a high energy acyl phosphate molecule from a low energy carboxylate, the cell must ‘spend’ the energy of one ATP molecule. The hydrolysis reaction is happening in the same enzyme active site – as the NH 3 is expelled in the hydrolysis of glutamine, it immediately turns around and acts as the nucleophile in the conversion of aspartyl-AMP to asparagine:

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