Next, a kind of acyl substitution occurs that we have not yet seen: the amine nitrogen on the aspartate attacks the electrophilic carbon of a carbon-nitrogen double bond, and the reaction proceeds thr...Next, a kind of acyl substitution occurs that we have not yet seen: the amine nitrogen on the aspartate attacks the electrophilic carbon of a carbon-nitrogen double bond, and the reaction proceeds through a tetrahedral intermediate before the AMP group is expelled, taking with it what was originally the carbonyl oxygen of the starting amide.
