In order to prevent the hydride transfer from proceeding, the researchers first hydrogenated the isolated C 2 -C 3 double bond on a sample of NADPH, using a palladium catalyst (under the conditions us...In order to prevent the hydride transfer from proceeding, the researchers first hydrogenated the isolated C 2 -C 3 double bond on a sample of NADPH, using a palladium catalyst (under the conditions used, the conjugated C 5 -C 6 double bond was left unreduced). The final step in the mechanism is where it gets really interesting: a hydride ion is transferred from the tetrahydrofolate coenzyme to the methylene (CH 2 ) group on the deoxynucleotide substrate.
