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2.5.6: Coloración de flagelos (tinción diferencial)

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    51409
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    Los flagelos bacterianos son estructuras de locomoción demasiado finas para visualizarlos con el microscopio óptico sin tinción. Para lograr su coloración se trata de engrosarlos aplicando soluciones coloidales inestables de ácido tánico, que se depositan sobre estas estructuras engrosando el diámetro aparente de los mismos, de tal manera que se hacen visibles al microscopio óptico con una tinción con fucsina básica (Figura 2.6.B). En la coloración de flagelos requiere mucho cuidado la fijación de la bacteria en la confección del preparado, dado la facilidad con que pueden desprenderse los flagelos por daños mecánicos.

    clipboard_ee672897ef63bec4f8b124df55440796d.png
    Figure \(\PageIndex{1}\): Tinción de flagelos. (CC-BY; This work)