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9.2: Fraccionamiento

Última actualización

29 oct 2022
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- 9.1: Interrupción celular
- 9.3: Cromatografía de intercambio iónico

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Figura 9.2.1: *Fraccionamiento por centrifugación*

Muchas técnicas cromatográficas se realizan en “columnas”. Se trata de tubos que contienen el material (llamado el “soporte”) utilizado para realizar la separación. Los soportes están diseñados para explotar las diferencias químicas, o de tamaño, de las muchas moléculas en una mezcla. Las columnas se “empaquetan” (rellenan) con el soporte y un tampón o disolvente lleva la mezcla de compuestos a separar a través del soporte. Las moléculas en la muestra interactúan diferencialmente con el soporte y consecuentemente, viajarán a través del mismo con diferentes velocidades.

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